Biofortification is increasingly seen as an additional tool to combat micronutrient malnutrition. This paper presents, for the first time, evidence on the costs and potential benefits of biofortification for a large number of countries in Africa, Asia and Latin America. We use a modification of the Disability-Adjusted Lif Years framework to conclude that the intervention can make a significant impact on the burden of micronutrient deficiencies in the developing world, and can do so in a highly cost-effective manner.